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Phospho-MAPK14-Y182 pAb (AP0057)

Review (2)Publication (1) Datasheet

ABclonal:Western blot - Phospho-MAPK14-Y182 pAb (AP0057)
ABclonal:Western blot - Phospho-MAPK14-Y182 pAb (AP0057)
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)
ABclonal:Immunofluorescence - Phospho-MAPK14-Y182 pAb (AP0057)
Review
ABclonal: review for Phospho-MAPK14-Y182 pAb(AP0057)
Review
ABclonal: review for Phospho-MAPK14-Y182 pAb(AP0057)

Overview

Product namePhospho-MAPK14-Y182 pAb
Catalog No.AP0057
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
ImmunogenA synthetic phosphorylated peptide around Y182 of human MAPK14 (NP_620581.1).
SequenceTGYVA
Gene ID1432
Swiss protQ16539
SynonymsMAPK14; CSBP; CSBP1; CSBP2; CSPB1; EXIP; Mxi2; PRKM14; PRKM15; RK; SAPK2A; p38; p38ALPHA; p38 MAPK
Calculated MW29kDa/34kDa/35kDa/41kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesNIH/3T3, 3T3L1
Cellular locationCytoplasm, Nucleus
Customer validation

(, )

WB(Human)

ABclonal:Western blot - Phospho-MAPK14-Y182 pAb (AP0057) }

Western blot - Phospho-MAPK14-Y182 pAb (AP0057)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-p38 MAPK-Y182 pAb (AP0057) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - Phospho-MAPK14-Y182 pAb (AP0057) }

Western blot - Phospho-MAPK14-Y182 pAb (AP0057)

Western blot analysis of extracts of 3T3L1 cells, using Phospho-MAPK14-Y182 pAb (AP0057) at 1:1000 dilution or p38 MAPK antibody (A10832).3T3L1 cells treated with different concentrations of Irisin.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057) }

Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)

Immunohistochemistry of paraffin-embedded rat lung using Phospho-MAPK14-Y182 antibody (AP0057) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057) }

Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)

Immunohistochemistry of paraffin-embedded rat liver using Phospho-MAPK14-Y182 antibody (AP0057) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057) }

Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)

Immunohistochemistry of paraffin-embedded rat testis using Phospho-MAPK14-Y182 antibody (AP0057) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057) }

Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)

Immunohistochemistry of paraffin-embedded rat ovary using Phospho-MAPK14-Y182 antibody (AP0057) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057) }

Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)

Immunohistochemistry of paraffin-embedded rat spleen using Phospho-MAPK14-Y182 antibody (AP0057) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057) }

Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)

Immunohistochemistry of paraffin-embedded human lung using Phospho-MAPK14-Y182 antibody (AP0057) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057) }

Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)

Immunohistochemistry of paraffin-embedded human colon using Phospho-MAPK14-Y182 antibody (AP0057) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057) }

Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)

Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-MAPK14-Y182 antibody (AP0057) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057) }

Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)

Immunohistochemistry of paraffin-embedded human appendix using Phospho-MAPK14-Y182 antibody (AP0057) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057) }

Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)

Immunohistochemistry of paraffin-embedded human breast cancer using Phospho-MAPK14-Y182 antibody (AP0057) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057) }

Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)

Immunohistochemistry of paraffin-embedded human placenta using Phospho-MAPK14-Y182 antibody (AP0057) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057) }

Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)

Immunohistochemistry of paraffin-embedded human stomach using Phospho-MAPK14-Y182 antibody (AP0057) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057) }

Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)

Immunohistochemistry of paraffin-embedded human gastric cancer using Phospho-MAPK14-Y182 antibody (AP0057) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057) }

Immunohistochemistry - Phospho-MAPK14-Y182 pAb (AP0057)

Immunohistochemistry of paraffin-embedded mouse testis using Phospho-MAPK14-Y182 antibody (AP0057) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Phospho-MAPK14-Y182 pAb (AP0057) }

Immunofluorescence - Phospho-MAPK14-Y182 pAb (AP0057)

Immunofluorescence analysis of U2OS cells using Phospho-MAPK14-Y182 antibody (AP0057). Blue: DAPI for nuclear staining.

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